Heterozygous osteopetrotic (op) mutation reduces atherosclerosis in LDL receptor-deficient mice

Abstract

Previous studies of osteopetrotic (op) mice lacking macrophage colony- stimulating factor (M-CSF) have revealed an inhibition of atherosclerosis development in the apolipoprotein E (apo E)-deficient model and in a diet- induced model. Using LDL receptor-deficient mice, we now show that atheroma development depends on M-CSF concentration, as not only did homozygous osteopetrotic (op/op) mice have dramatically reduced lesions (~ 0.3% of control lesion size) but heterozygous (opl+) mice had lesions < 1% of controls. Mice heterozygous for the op mutation (opl+) had plasma levels of M-CSF about half those in controls (+/+). The finding that an ~ 2-fold reduction in M-CSF expression reduced lesion size ~ 100-fold suggests the requirement for a threshold level of M-CSF. The effect of M-CSF on atherosclerosis did not appear to be mediated either by changes in plasma lipoprotein levels or alterations in the number of circulating monocytes, since both op/op and op/+ mice exhibited higher levels of atherogenic lipoprotein particles and (op/+) mice showed a near normal number of circulating monocytes. LDL receptor-null littermates of genotypes from op/op, op/+, to +/+ showed monocyte differentials of ~ 4.5, 8, and 10%, respectively. Taken together, these results suggest that the effects of M- CSF on atherogenesis may not be mediated by expression of M-CSF systemically or by modulation of the number of circulating monocytes. These studies support the conclusion that M-CSF participates critically in fatty streak formation and progression to a complex fibrous lesion.