We introduce Cryogenic Optical Localization in 3D (COLD), a method to localize multiple fluorescent sites within a single small protein with Angstrom resolution. We demonstrate COLD by determining the conformational state of the cytosolic Per-ARNT-Sim domain from the histidine kinase CitA of Geobacillus thermodenitrificans and resolving the four biotin sites of streptavidin. COLD provides quantitative 3D information about small- to medium-sized biomolecules on the Angstrom scale and complements other techniques in structural biology.
CITATION STYLE
Weisenburger, S., Boening, D., Schomburg, B., Giller, K., Becker, S., Griesinger, C., & Sandoghdar, V. (2017). Cryogenic optical localization provides 3D protein structure data with Angstrom resolution. Nature Methods, 14(2), 141–144. https://doi.org/10.1038/nmeth.4141
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