Cloning and expression of an Endo-1,6-β-D-glucanase gene (neg1) from neurospora crassa

Abstract

A gene (neg1) encoding an endo-1,6-β-D-glucanase from Neurospora crassa was cloned. The putative neg1 was 1443-bp long and encoded a mature endo-1,6-β-D-glucanase protein of 463 amino acids and signal peptide of 17 amino acids. The purified recombinant protein (Neg1) obtained from Escherichia coli showed 1,6-β-D-glucanase activity. No genes similar in sequence were found in yeasts and fungi. © 2002 by Japan Society for Bioscience, Biotechnology, and Agrochemistry.