Skip to main content

Increased cutaneous absorption reflects impaired barrier function of reconstructed skin models mimicking keratinisation disorders

13Citations
Citations of this article
27Readers
Mendeley users who have this article in their library.

This artice is free to access.

Abstract

The aim of this study was to assess a recently established 3D model of congenital ichthyosis, representing severe epidermal barrier function defects, for skin penetration and permeation. We have generated disease models by knock-down of either TGM1 or ALOXE3 in primary human keratinocytes, and using keratinocytes and fibroblasts from patients with congenital ichthyosis. The results indicate disturbed barrier function as demonstrated by increased permeation of testosterone and caffeine particularly in TGM1 knock-down models compared to control models. In addition, enhanced penetration of the model dye nile red incorporated into solid lipid nanoparticles and core-multishell nanotransporters, respectively, was evident in disease models. Thus, in vitro skin disease models reproduce differences in barrier permeability and function seen in congenital ichthyosis and pave the way to personalised disease models. Furthermore, our findings indicate that nanocarriers may be useful in new, topical therapeutic approaches for the currently very limited treatment of congenital ichthyosis. © 2014 John Wiley & Sons A/S.

Cite

CITATION STYLE

APA

Eckl, K. M., Weindl, G., Ackermann, K., Küchler, S., Casper, R., Radowski, M. R., … Schäfer-Korting, M. (2014). Increased cutaneous absorption reflects impaired barrier function of reconstructed skin models mimicking keratinisation disorders. Experimental Dermatology, 23(4), 286–288. https://doi.org/10.1111/exd.12366

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free