Investigation of enterobacter aerogenes effects on heavy oil from biological degradation aspects by GC*GC technique

Abstract

The main cause of oil pollution of soil is the emergency situations within oil production, transportation and processing in the boundaries of industrial sites of chemical and petrochemical industries. Oil pollution leads to the deterioration of the agrophysics soil characteristics, namely to the dysfunction of the water, air, thermal, oxidation-reduction and nutrient regimens. Microbial technologies are becoming accepted worldwide as cost-effective and environmentally friendly approaches to improve oil production. One of the methods to purify oil residue is bioremediation, in this way we Investigate Enterobacter Aerogenes effects on heavy oil by SARA, FT-IR, GC and GCxGC methods. The material required for the growth of Enterobacter Aerogenes is Carbon and Phosphate. On the one hand, this bacterium takes these nutrients from carbohydrates in our body. In addition, the crude oil is full of Hydrocarbon structures. So, the Enterobacter Aerogenes is injected with Thioglycollate broth to crude oil which was distillated and the samples were put for a month in an incubator for 30 days. The results this research illustrate this bacterium has been able to survive in oil by isolating straight-run carbons with short and weak bounds from asphaltene and resin structures, as well as utilizing sulfur, nitrogen and oxygen in the oil. Also, it is found out this bacterium has had a positive effect on making light oil from heavy crude oil. It should be noted this bacterium releases CH4, which can be used again if the gas is collected.