The rat is an important animal model to understand gene function and model human diseases. Since recent years, the development of gene-specific nucleases has become important for generating new rat models of human diseases, to analyze the role of genes and to generate human antibodies. Transcription activator-like (TALE) nucleases efficiently create gene-specific knockout rats and lead to the possibility of gene targeting by homology-directed recombination (HDR) and generating knock-in rats. We describe a detailed protocol for generating knockout and knock-in rats via microinjection of TALE nucleases into fertilized eggs. This technology is an efficient, cost-and time-effective method for creating new rat models
CITATION STYLE
Tesson, L., Remy, S., Ménoret, S., Usal, C., Thinard, R., Savignard, C., … Anegon, I. (2016). Genome editing in rats using TALE nucleases. In Methods in Molecular Biology (Vol. 1338, pp. 245–259). Humana Press Inc. https://doi.org/10.1007/978-1-4939-2932-0_18
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