Pulsed-field gel electrophoresis (PFGE) is a technique for resolving large (up to 10 Mb) DNA molecules. Using multiple pairs of electrodes DNA is subject to an alternating electric field through a solid agarose matrix. As the current changes direction the reorientation time of DNA is proportional to molecular weight; thus fragments are separated in the gel based on their size. Here we describe the use of PFGE to analyze DNA double-strand break formation and repair in human chromosomal DNA. © 2012 Springer Science+Business Media New York.
CITATION STYLE
Bryant, H. E. (2012). DNA double-strand break damage and repair assessed by pulsed-field gel electrophoresis. Methods in Molecular Biology, 920, 315–321. https://doi.org/10.1007/978-1-61779-998-3_22
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