Assays for Thiols and Modifications

Abstract

This chapter comments on how to assay thiols and their modifications. Several biological compounds are derived from cysteine (the main cellular thiol), cysteamine, glutathione (GSH, gamma-glutamyl-cysteinyl-glycine), coenzyme A, and their disulfide and mixed disulfide forms, such as cystine. In addition, there are other biological sulfur compounds such as hydrogen sulfide, methionine, and polysulfur molecules. This chapter focuses on cysteine, glutathione, and their oxidized and S-conjugated forms including the S-nitrosated products of cysteine and glutathione.