Glu-44 in the amino-terminal α-Helix of yeast vacuolar atpase e subunit (Vma4p) has a role for VoV1 assembly

Abstract

Background: The V-ATPase is inactivated by reversible disassembly upon glucose starvation. Results: Glu-44 of the V-ATPase E subunit was identified by alanine scanning mutagenesis with reduced disassembly under glucose starvation. Conclusion: Mutations to Glu-44 of the E subunit disrupt normal disassembly of the V-ATPase and affect catalytic activity. Significance: Interactions with the E subunit in the V-ATPase are important for controlling disassembly and catalytic activity. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc. Published in the U.S.A.