Proline requirement for glucose utilization by Peptostreptococcus anaerobius ATCC 27337

Abstract

Resting cells of Peptostreptococcus anaerobius maintained under anaerobic conditions were unable to metabolize either glucose or alanine. The addition of proline to the appropriate suspension, however, resulted in the immediate utilization of both compounds. Fermentation of alanine by the cells required that stoichiometric concentrations of proline be present in the medium; and during the oxidation of alanine, proline was simultaneously reduced to the ring cleavage product δ-aminovaleric acid. Although proline was required to initiate glucose transport, stoichiometric amounts of the imino acid were not necessary for glucose fermentation. Proline also stimulated the uptake and concomitant phosphorylation of the nonmetabolizable glucose analog 2-deoxy-D-glucose. The proline requirement for glucose transport by P. anaerobius could be replaced by adding ferricyanide or simply by aerating the cell suspension. The initiation of sugar uptake by proline, ferricyanide, and O2 was attributed to the capacity of these compounds to function as electron acceptors, which permitted (i) reoxidation of the (reduced) intracellular nucleotide pool and (ii) the formation (from an endogenous reserve) of the high-energy donor(s) required for the vectorial transport and phosphorylation of sugar.