Laser Interferometry Analysis of Ciprofloxacin Diffusion through Pseudomonas aeruginosa Biofilm

Abstract

In this study the novel application of laser interferometry method in quant. anal. of drugs distributions in bacterial biofilms is presented. Ciprofloxacin diffusion through P. aeruginosa PAO1 biofilm was used as a model system. It was detd. that 0.759 μmol (25.32%) of ciprofloxacin was transported through biofilm after 2400 s from initial amt. of 3 μmol. Addnl., laser interferometry method was used to calc. the amt. (mol) of drug accumulated in the biofilm formed on nucleopore membrane. We obsd. that the amt. of ciprofloxacin into biofilm is 0.366 μmol (12.2%) after 2400 s. These results were in accordance with measurements obtained by std. cultivation methods. In conclusion, laser interferometry technique might be useful tool in real time calcn. of drug concn. in bacterial biofilm as well as its transport through that structure. From clin. point of view, this important information might be used in modeling of antibiotics distribution in correlation with their biol. effects on bacterial biofilms. [on SciFinder(R)]