This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging.
CITATION STYLE
Gratton, E., & vandeVen, M. J. (1995). Laser Sources for Confocal Microscopy. In Handbook of Biological Confocal Microscopy (pp. 69–97). Springer US. https://doi.org/10.1007/978-1-4757-5348-6_5
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