Screening of the antifungal potential of nudibranch Chromodoris lineolata associated bacteria against Candida albicans

Abstract

Skin disease is the fourth most common disease caused by infection of opportunistic pathogens on the skin. Skin diseases are generally treated by antibiotic products. However, overuse of antibiotics can increase the resistance of pathogens to various classes of antibiotics. Therefore, the study of new antimicrobial compounds against skin pathogens is urgently needed. This study aims to isolate nudibranch Chromodoris lineolata associated bacteria with antifungal activity against Candida albicans and detect the presence of type I polyketide synthase (PKS I), type II polyketide synthase (PKS II), and non-ribosomal peptide synthetase (NRPS) genes through a molecular approach. A total of 15 bacteria were successfully isolated from nudibranch at Panjang Island and Teluk Awur. Screening of the antifungal activity using an agar plug method showed that 1 isolate namely P.10.6 showed antifungal activity against Candida albicans. The detection of the biosynthetic gene cluster (BGC) showed the absence of BGC in the potential isolate. This finding was suspected due to the production of the antifungal compound from the other biosynthetic pathways. Based on molecular identification through BLAST homology, strain P. 10.6 has been identified as Bacillus stratosphericus.