Abstract
The chapter will focus on the high level cell-free production of integral membrane proteins having multiple transmembrane segments by using an individual coupled transcription/translation system based on an Escherichia coli S30-extract. We describe in detail the setup and optimization of the cell-free expression technique to obtain the maximum yield of recombinant proteins. The protocol can be used for the expression of soluble membrane proteins as well as for their production as a precipitate. In addition, we will provide protocols for the efficient solubilization and reconstitution of membrane proteins directly from the cell-free produced precipitates.
Cite
CITATION STYLE
Klammt, C., Schwarz, D., Dötsch, V., & Bernhard, F. (2007). Cell-Free Production of Integral Membrane Proteins on a Preparative Scale. In In Vitro Transcription and Translation Protocols (pp. 57–78). Humana Press. https://doi.org/10.1007/978-1-59745-388-2_3
Register to see more suggestions
Mendeley helps you to discover research relevant for your work.
