Genetic diversity of regeneration ability in anther culture of rice (Oryza sativa L.)

Abstract

Rice (Oryza sativa L.) cv. Asahi has been proved to be a useful genetic resource with a unique regeneration ability in anther culture. Diallel analysis was employed using four varieties showing different responses to four different constituent media. The mode of inheritance in regeneration ability of Asahi changed between dominant and recessive expression depending on medium composition although its regeneration rate was constantly high. On the contrary, the low regeneration ability of Koshihikari behaved consistently in a dominant manner and the high regeneration ability of Tsukinohikari behaved in a recessive manner independent of medium composition. The basic medium used was N6 medium containing 0.1μM 2,4-D, 5μM NAA and 70 g L--1 sucrose and the others were supplemented with 0.1μM BAP, 0.1μM BAP+18 mg L-1 glycine or 0.1μM BAP+18 mg L-1 glycine+and 0.5 g L-1 alanine. The difference of medium composition which affects dominance and recessiveness in regeneration ability of Asahi was affected by the presence or absence of 18 mg L-1 glycine supplemented in the medium.