Tissue printing and dot blot are simple techniques to detect miRNA expression and localization, allowing a better understanding of the function of a miRNA. In this work, we describe a tissue printing and a dot blot hybridization protocol for miRNA detection and localization in plant tissues, which opens the possibility of analyzing spatiotemporal expression patterns of miRNAs.
CITATION STYLE
Martínez Núñez, M., de Folter, S., & Rosas-Cárdenas, F. de F. (2019). Detection of miRNAs by tissue printing and dot blot hybridization. In Methods in Molecular Biology (Vol. 1932, pp. 151–157). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9042-9_11
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