A comparative study on meiotic competence of pig oocyte by two methods for collection: Aspiration and dissection

Abstract

The aim of this study is to examine the effect of different collection technique to the developmental meiotic competent after in vitro maturation (IVM). Ovaries were collected from local adult and non-pregnant slaughtered pig. Porcine oocytes were collected by both aspiration method and dissection technique. Follicle isolated from dissection are classified into 3 groups: Group 1: 3–4 mm, Group 2: 4–5 mm, Group 3: 5–6 mm. Oocytes from aspiration technique take place as another group. These groups were cultured in maturation medium for 40–42 h, at 38.5 °C, 5% CO2. After maturation process, the meiotic competence of oocytes was examined by orcein staining. The results show that the percentages of oocyte reach to metaphase II from Group 3 (68.19%), Group 2 (52.12%) and Aspiration group (39.2%) are significantly higher than Group 1 (7.15%), especially, Group 3 is significantly higher than the Aspiration group. Therefore, it is the most effective way to use oocytes from follicles of 4–6 mm in diameter, especially 5–6 mm, for IVM process in experimental research and subsequent technology. We suggest that the oocytes from Group 1 (follicle size 3–4 mm) are needed to pass a pre-IVM or incubate in IVG for a short time before introducing to IVM process.