Urinary Elimination and Metabolism of Nitrosamines in Different Dietary Protein Wistar Rat Models

Abstract

It is necessary to establish comparatively whether or not there are differences in the mode of elimination of Nitrosodimethylamine (NDMA) and /or Aflatoxin B1 (AFB1) in urine as between different nutritional states of dietary protein. Four groups of albino wistar rats were fed protein free, normal protein repletion, low protein and high protein diets. NDMA was excreted in urine of rats placed on protein free diet at higher quantity (27.19%) than protein repleted rats (19.35%) and in low protein fed rats at a lower amount (22.82%) than in high protein fed rats (32.68%,) after NDMA was given. There was a lower urinary excretion of NDMA in protein free fed rats (22.18%) than in their protein repleted counterparts (33.77%) and low protein fed rats excreted more NDMA (27.14%) than those placed on high protein (11.79%), after a concurrent dose of NDMA and AFB1. It appears the elimination of NDMA is enhanced by protein free intake compared with normal protein repletion intake while it was reduced by low protein intake as compared to high protein intake. AFB1 increased the elimination of NDMA in normal protein repletion and low protein intakes whereas decreased NDMA elimination was observed for protein free and high protein intakes.