The experimental conditions of nonenzymatic reactivation of des-acetyl citrate lyase from K. aerogenes were studied. It was found that at pH 8.5 0.2 MM acetyl AMP causes a fast reactivation of the enzyme. The pH dependence of activity and the Km for citrate are very similar for both native and reactivated enzyme.
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Marcantonio, M., Chiarucci, A., Maccherini, S., Guglietta, D., & Bacaro, G. (2012). Plant biodiversity of beech forests in central-northern Italy: a methodological approach for conservation purposes. Forest@ - Rivista Di Selvicoltura Ed Ecologia Forestale, 9(4), 198–216. https://doi.org/10.3832/efor0698-009
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