Growth and Enzyme Production of Proteolytic Bacteria from Mangrove Sediment

Abstract

Accumulation of organic component in mangrove ecosystem results in diversity of microorganisms producing extracellular enzymes. Two species of proteolytic bacteria had been isolated and identified from sediment of mangrove ecosystem in the Dumai Marine Station of Riau Province, Indonesia. This research aimed to assess the activity and enzyme production of the proteolytic bacteria, i.e. Bacillus manliponensis (isolate code P.Az6) and B. toyonensis (P.Az20). The growth of bacteria in tryptic soy broth (TSB + skimmed milk) was observed by using spectrophotometer at λ 610 nm and by analysis the total plate counts on Zobell Marine Agar at 0, 6, 12, 18 and 24 hours. Production of enzyme protease was measured from the absorbance values of bacterial supernatant, then were conversed to the protease concentration by using the Bovine serum Albumin Standard. The highest bacterial growth and optimal enzyme production was observed at 6 hours incubation, and period after that showed decrease in growth enzymatic activity. Bacillus toyonensis showed higher bacterial growth and enzyme production than B. manliponensis.