The investigation of cellular processes on the molecular level is important to understand the functional network within plant cells. self-assembling GFP has evolved to be a versatile tool for (membrane) protein analyses. Based on the autocatalytical reassembling property of the nonfluorescent strands 1-10 and 11, protein distribution and membrane protein topology can be analyzed in vivo. Here, we provide basic protocols to determine membrane protein topology in Arabidopsis thaliana protoplasts. © 2013 Springer Science+Business Media, LLC.
CITATION STYLE
Wiesemann, K., Groß, L. E., Sommer, M., Schleiff, E., & Sommer, M. S. (2013). Self-assembling GFP: A versatile tool for plant (membrane) protein analyses. Methods in Molecular Biology, 1033, 131–144. https://doi.org/10.1007/978-1-62703-487-6_9
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