Comparison of various carbohydrates for hydrogen production in microbial electrolysis cells

Abstract

The direct production of hydrogen from various carbohydrates of lignocellulosic biomass is examined by using single-chamber membrane-free microbial electrolysis cells. The mixed microbial culture was enriched with 20 mmol L-1 2-chloroethane sulphonate and 10 mmol L-1 sodium acetate as a carbon source. Hydrogen was produced from all carbon sources used, including monosaccharides (glucose, galactose, mannose, xylose and arabinose) and disaccharides (maltose, saccharose and cellobiose). Hydrogen gas (18 mL) was produced using 3.33 mmol L-1 maltose and 20 mL of hydrogen was produced using cellobiose after 14 d of operation. Methane was not produced under any of the tested conditions. The effects of sugar concentration were also examined. Hydrogen production rates varied from 0.01 m3 d-1 m-3 to 0.09 m3 d-1 m-3. The maximum hydrogen yield ranged from 14% to 111%. Our results suggested that carbohydrates, such as maltose and cellobiose, are suitable carbon sources for hydrogen production, when methanogens are suppressed.