Blot overlay is a useful method for studying protein-protein interactions. This technique involves fractionating proteins on SDS-PAGE, blotting to nitrocellulose or PVDF membrane, and then incubating with a probe of interest. The probe is typically a protein that is radiolabeled, biotinylated, or simply visualized with a specific antibody. When the probe is visualized via antibody detection, this technique is often referred to as “Far Western blot.” Many different kinds of protein-protein interactions can be studied via blot overlay, and the method is applicable to screens for unknown protein-protein interactions as well as to the detailed characterization of known interactions.
CITATION STYLE
Hall, R. A. (2015). Studying protein-protein interactions via blot overlay/far western blot. Methods in Molecular Biology, 1278, 371–379. https://doi.org/10.1007/978-1-4939-2425-7_24
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