Periodic Acid-Schiff (PAS) with diastase (PAS-D) refers to the use of the PAS stain in combination with diastase, which is an enzyme that digests the glycogen. The purpose of using the PAS-D procedure is to differentiate glycogen from other PAS-positive elements in tissue samples. The PAS-D method is also used for periportal liver staining of AAT polymer inclusions that are seen in alpha-1 antitrypsin deficiency disease. Here, we describe the procedure of PAS-D staining in formalin-fixed, paraffin-embedded human liver tissues.
CITATION STYLE
Fu, D. A., & Campbell-Thompson, M. (2017). Periodic acid-schiff staining with diastase. In Methods in Molecular Biology (Vol. 1639, pp. 145–149). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7163-3_14
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