Saturation transfer MRI is sensitive to neurochemical changes in the rat brain due to chronic unpredictable mild stress

0Citations
Citations of this article
8Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Chemical exchange saturation transfer (CEST) MRI was performed for the evaluation of cerebral metabolic changes in a rat model of depressive-like disease induced by chronic unpredictable mild stress (CUMS). CEST Z-spectra were acquired on a 7 T MRI with two saturation B1 amplitudes (0.5 and 0.75 µT) to measure the magnetization transfer ratio (MTR), CEST and relayed nuclear Overhauser effect (rNOE). Cerebral cortex and hippocampus were examined in two groups of animals: healthy control (n = 10) and stressed (n = 14), the latter of which was exposed to eight weeks of the CUMS protocol. The stressed group Z-spectrum parameters, primarily MTRs, were significantly lower than in controls, at all selected frequency offsets (3.5, 3.0, 2.0, − 3.2, − 3.6 ppm) in the cortex (the largest difference of ~ 3.5% at − 3.6 ppm, p = 0.0005) and the hippocampus (MTRs measured with a B1 = 0.5 µT). The hippocampal rNOE contributions decreased significantly in the stressed brains. Glutamate concentration (assessed using ELISA) and MTR at 3 ppm correlated positively in both brain regions. GABA concentration also correlated positively with CEST contributions in both cerebral areas, while such correlation with MTR was positive in hippocampus, and nonsignificant in cortex. Results indicate that CEST is sensitive to neurometabolic changes following chronic stress exposure.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Pankowska, A., Chudzik, A., Słowik, T., Łazorczyk, A., Kochalska, K., Andres-Mach, M., … Orzyłowska, A. (2021). Saturation transfer MRI is sensitive to neurochemical changes in the rat brain due to chronic unpredictable mild stress. Scientific Reports, 11(1). https://doi.org/10.1038/s41598-021-97991-0

Readers over time

‘21‘22‘23‘2400.751.52.253

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 2

50%

Researcher 2

50%

Readers' Discipline

Tooltip

Medicine and Dentistry 2

40%

Neuroscience 2

40%

Chemistry 1

20%

Save time finding and organizing research with Mendeley

Sign up for free
0