Reprogramming of somatic cells to induced pluripotent stem (iPS) cells can be achieved by the delivery of a combination of transcription factors, including Oct4, Sox2, Klf4, and c-Myc. Retroviral and lentiviral vectors are commonly used to express these four reprogramming factors separately and obtain reprogrammed iPS cells. Although efficient and reproducible, these approaches involve the time-consuming and labor-intensive production of retroviral or lentiviral particles together with a high risk of working with potentially harmful viruses overexpressing potent oncogenes, such as c-Myc. Here, we describe a simple method to produce bona fide iPS cells from human fibroblasts using poly-β-amino esters as the transfection reagent for the delivery of a single CAG-driven polycistronic plasmid expressing Oct4, Sox2, Klf4, c-Myc, and a GFP reporter gene (OSKMG).Wedemonstrate for the first time that poly-β-amino esters can be used to deliver a single polycistronic reprogramming vector into human fibroblasts, achieving significantly higher transfection efficiency than with conventional transfection reagents. After a protocol of serial transfections using poly-β-amino esters, we report a simple methodology to generate human iPS cells from human fibroblasts avoiding the use of viral vectors. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.
CITATION STYLE
Montserrat, N., Garreta, E., González, F., Gutiérrez, J., Eguizábal, C., Ramos, V., … Belmonte, J. C. I. (2011). Simple generation of human induced pluripotent stem cells using poly-β-amino esters as the non-viral gene delivery system. Journal of Biological Chemistry, 286(14), 12417–12428. https://doi.org/10.1074/jbc.M110.168013
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