Chemical Constituents From the Vietnamese Medicinal Plant Anemone chapaensis and Their Cytotoxic Activity

Abstract

Our current research on phytochemical profile of the folk medicinal plant Anemone chapaensis Gagnep. led to the isolation of a new saponin, chapaenoside (1), along with 13 known compounds (2-14). Their structures were elucidated on the basis of extensive chemical and spectroscopic methods (Nuclear Magnetic Resonance [NMR] spectroscopy and mass spectrometry) as well as comparison with the literature data. The isolated compounds were tested for their cytotoxic activities against 9 cancer cell lines by MTT method. The result indicated that the major saponin prosapogenin CP6 (5) showed selectively strong activity against NCI-N87, RD, Panc-1, and MIA Paca-2 cells with the IC50 values of 5.4, 7.5, 7.5, and 2.7 μM, respectively. The results of phytochemical constituents and principal bioactive saponins from A. chapaensis contributed not only to its phytochemical profile associated with chemotaxonomy but also to biological evidence of the title plant.