Downregulation of microRNA-210 inhibits osteosarcoma growth in vitro and in vivo

Abstract

MicroRNA-210 (miR-210), the master hypoxamir, has various roles in the development of certain cancer types. It has been reported that miR-210 expression was upregulated in patients with osteosarcoma (OS). However, little is known regarding its role in the development of human OS. In the present study, to explore the feasibility of miR-210 as an effective therapeutic target, miR-210 inhibitor was transfected into the osteosarcoma cell line MG-63 cells, and cell proliferation, colony formation, cycle, apoptosis, migration and invasion were assessed. It was found that miR-210 downregulation signifi cantly suppressed clonogenicity, migration and invasion, as well as induced cell apoptosis, increased the percentage of cells in G1 phrase and decreased the percentage of cells in S phase in vitro. In addition, the effect of miR-210 on tumor growth was evaluated in vivo. The results indicated that miR-210 downregulation significantly suppressed tumor growth in nude mouse models. In conclusion, the findings of the present study suggested that miR-210 is a potential therapeutic agent for the treatment of OS.