Abstract
Hoffman microscopy imaging systems are part of numerous fertility clinics world-wide. We discuss the physics of the Hoffman imaging system from optical thickness to image intensity, implement a simple, yet fast, reconstruction algorithm using Fast Fourier Transformation and discuss the usability of the method on a number of cells from a human embryo. Novelty is identifying the non-linearity of a typical Hoffman imaging system, and the application of Fourier Transformation to reconstruct the optical thickness. © Springer-Verlag 2003.
Cite
CITATION STYLE
Olsen, N. H., Sporring, J., Nielsen, M., Hnida, C., & Ziebe, S. (2003). Reconstructing the optical thickness from hoffman modulation contrast images. Lecture Notes in Computer Science (Including Subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics), 2749, 526–533. https://doi.org/10.1007/3-540-45103-x_70
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