Application of Sol-Gel Method for Synthesis of a Biosensitive Polysiloxane Matrix

Abstract

Sol-gel method was used to synthesize polysiloxane hydrogel with encapsulated urease (immobilization degree in the range of 79–88%) which preserved the enzymatic activity at the level of 56–84%. The nature of functional groups was shown to influence the pore structure parameters. Immobilization degree and preservation of adsorbed urease activity depend on the structural-adsorption characteristics of matrices. The possibility of “double immobilization” of urease on silica gel by sol-gel method and the opportunity of reuse of the synthesized formulations was investigated. Urease immobilization on the surface of magnetite (FeO·Fe2O3) was also studied by adsorption method.