Molecular farming using bioreactor-based plant cell suspension cultures for recombinant protein production

Abstract

The need for biomanufacturing capacity for recombinant protein production to meet the expanding pharmaceutical and industrial market demands has gained increasing importance, leading to the development of new protein expression platforms capable of addressing requirements in terms of protein yield, product quality, and production cost. In the past few decades, molecular farming using plant -cell-based expression systems (whole plants and in vitro plant cells, organ and tissue cultures) have been investigated as an alternative for the large-scale bioproduction of recombinant proteins. Molecular farming using bioreactor-based plant cell suspension cultures provides attractive features over recombinant microbial fermentation and mammalian cell cultures in terms of intrinsic safety, cost-effective biomanufacturing, and the capability for post-translation modifications. The current research and development, emerging techniques, commercialization and future prospects of mole-cular farming using bioreactor-based plant cell suspension cultures for production of recombinant proteins will be discussed in this chapter.