PCR-based DNA array technology is one of the most suitable techniques to detect and identify multiple pathogens in a single assay. Out of the different array platforms that currently exist, membrane-based DNA macroarrays are the most convenient for plant disease diagnosis because of low costs, great sensitivity, and modest equipment requirements. Here we describe a protocol for routine detection of plant pathogens using DNA macroarrays, i.e., from sampling to analysis of hybridization results. Diagnosis can be completed within 36 h after sample collection. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Lievens, B., Justé, A., & Willems, K. A. (2012). Fungal plant pathogen detection in plant and soil samples using DNA macroarrays. Methods in Molecular Biology, 835, 491–507. https://doi.org/10.1007/978-1-61779-501-5_30
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