The controlled self-assembly of protein cages is vital for the use of these nanocompartments in biomedical and nanotechnological applications. Recently, we showed that by combining different structural peptide elements, it is possible to assemble viral capsid proteins in distinct well-defined morphologies. In this chapter, a triblock copolypeptide is discussed, consisting of a metal ion-coordinating hexahistidine tag, a stimulus-responsive elastin-like polypeptide and a pH-responsive self-assembling viral capsid protein. This protein is able to form two different types of capsids, depending on the assembly pathway that is followed. Here, we focus on the metal ion-induced assembly process and describe the relevant experimental procedures to induce and utilize this assembly behavior.
CITATION STYLE
Schoonen, L., van Eldijk, M. B., & van Hest, J. C. M. (2018). Metal ion-induced self-assembly and packaging of CCMV nanocapsules. In Methods in Molecular Biology (Vol. 1798, pp. 57–67). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7893-9_5
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