Production of exo-pectinase by Penicillium roqueorti using pumpkin oil cake

Abstract

Submerged fermentation (SmF) was carried out using the pumpkin oil cake (PuOC) as a substrate for the production of exo-pectinase (exo-p) by Penicillium roqueforti. PuOC was used as carbon, nitrogen and mineral source for fungi growth and exo-p production. SmF was carried out in the production medium containing 0.2% KH2PO4, 0.1% MgSO4 and 0.1% NaCl, supplemented with PuOC at 2% concentration. The obtained results show that P. roqueforti cultivated in a liquid medium in the presence of PuOC as a source of nitrogen, produced significant amount of exo-p, activity 161.03 U/ml.Cilj ovog rada je bio istrazivanje mikrobioloske konverzije i revalorizacije bundevine uljane pogace (PuOC) pomocu biotehnoloskih postupaka. Izvedena je submerzna kultivacija pomocu plesni Penicillium roqueforti (GRAS status), a u cilju dobijanja egzo-pektinaza (egzo-p). Submerzna fermentacija (SmF) izvedena je u tecnoj podlozi sa 0.2% KH2PO4, 0.1% MgSO4 i 0.1% NaCl, u koju je dodato 2% PuOC. Dobijeni razultati pokazuju da je produkcija egzo-p pomocu P. roqueforti u ovakvoj podlozi moguca, da se enzim pokoreva dvostepenoj kinetici i da ima prvi maksimum aktivnosti, vrednosti 111.75 U/ml, treceg dana kultivacije i drugi maksimum, cija je vrednost 161.03 U/ml, sestog dana procesa.