Many knock-out/knock-in mouse and rat strains have been produced by genome editing techniques using engineered endonucleases, including zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), or clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. Microinjection of engineered endonucleases into pronuclear-stage embryos is required to produce genome-edited rodents and the development of easy, rapid, and high-efficiency methods that do not require special skills such as microinjection is needed. This chapter presents a new technique called Technique for Animal Knockout system by Electroporation (TAKE), which produces genome-edited rodents by direct introduction of engineered endonucleases into intact embryos using electroporation.
CITATION STYLE
Kaneko, T. (2017). Genome editing in mouse and rat by electroporation. In Methods in Molecular Biology (Vol. 1630, pp. 81–89). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7128-2_7
Mendeley helps you to discover research relevant for your work.