Determining oxidative stress of spermatozoa

Abstract

Oxidative stress is triggered via numerous reactive oxygen species like peroxides, superoxide anions, nitric oxide, hydroxyl radical and singlet oxygen, which any biological cell/ system is unable to counterbalance. In assisted reproductive technologies, the status of seminal oxidative stress can be significantly applied for diagnosing a malady along with prophylactic measures. Accurate oxidative stress measurement may help to develop strategies to reduce oxidative stress during extension and cryopreservation of bovine semen. The present chapter outlines different procedures of measuring oxidative stress in semen samples and their comparative analyses.