Thiol-ene photoimmobilization of chymotrypsin on polysiloxane gels for enzymatic peptide synthesis

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Abstract

Chemical incorporation of enzymes onto polymeric materials has recently attracted intense scientific attention. Cross-linked polysiloxane gels as a typical super-hydrophobic support, are a good candidate for supporting enzymes in low-water organic medium to efficiently catalyze peptide synthesis because the hydrophobic polysiloxane matrix can prevent water from attacking the acyl-enzyme intermediate, which is beneficial for the shift in equilibrium to peptide formation. In this work, we develop a facile strategy to photoimmobilize olefin-functionalized chymotrypsin onto cross-linked polysiloxane gels via UV-initiated thiol-ene click chemistry. The impacts of water addition amount, heat-treatment and recyclability of the immobilized chymotrypsin influencing the peptide synthesis efficiency are investigated. Compared with the native chymotrypsin, polysiloxane-immobilized chymotrypsin showed advantageous catalytic activity, higher thermal stability and superior recyclability.

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Wang, M., Xing, J., Sun, Y. T., Guo, L. X., Lin, B. P., & Yang, H. (2018). Thiol-ene photoimmobilization of chymotrypsin on polysiloxane gels for enzymatic peptide synthesis. RSC Advances, 8(22), 11843–11849. https://doi.org/10.1039/c7ra13320k

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