Enhancement of homology-directed repair with chromatin donor templates in cells

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Abstract

A key challenge in precise genome editing is the low efficiency of homology-directed repair (HDR). Here we describe a strategy for increasing the efficiency of HDR in cells by using a chromatin donor template instead of a naked DNA donor template. The use of chromatin, which is the natural form of DNA in the nucleus, increases the frequency of HDR-edited clones as well as homozygous editing. In addition, transfection of chromatin results in negligible cytotoxicity. These findings suggest that a chromatin donor template should be useful for a wide range of HDR applications such as the precise insertion or replacement of DNA fragments that contain the coding regions of genes.

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Cruz-Becerra, G., & Kadonaga, J. T. (2020). Enhancement of homology-directed repair with chromatin donor templates in cells. ELife, 9. https://doi.org/10.7554/eLife.55780

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