ATP measurement in cerebrospinal fluid using a microplate reader

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Abstract

Imbalance in extracellular ATP levels in brain tissue has been suggested as a triggering factor for several neurological disorders. Here, we describe the most sensitive and reliable technique for monitoring the ATP levels in mice cerebrospinal samples collected by cisterna magna puncture technique and quantified using a microplate reader.

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de Diego-García, L., Sebastián-Serrano, Á., Bianchi, C., Di Lauro, C., & Díaz-Hernández, M. (2020). ATP measurement in cerebrospinal fluid using a microplate reader. In Methods in Molecular Biology (Vol. 2041, pp. 233–241). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9717-6_17

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