The opsonophagocytic killing assay (OPKA) is designed to measure the functionality of strain-specific antibodies and, therefore, assess protective immunity or the immunogenicity of Group A Streptococcus (GAS) (type A Streptococcus pyogenes) vaccines. Opsonization of GAS for phagocytosis is an important mechanism by which antibodies protect against disease in vivo. The Opsonophagocytic Index or Opsonic Index (OI) is the estimated dilution of antisera that kills 50% of the target bacteria. Here, we describe the protocol of the standardized GAS OPKA developed by Jones et al., 2018.
CITATION STYLE
Wagstaffe, H., Jones, S., Johnson, M., & Goldblatt, D. (2022). Opsonophagocytic Killing Assay to Measure Anti–Group A Streptococcus Antibody Functionality in Human Serum. In Methods in Molecular Biology (Vol. 2414, pp. 373–386). Humana Press Inc. https://doi.org/10.1007/978-1-0716-1900-1_20
Mendeley helps you to discover research relevant for your work.