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Development of randomly amplified polymorphic DNA based SCAR marker for identification of Ipomoea mauritiana Jacq (Convolvulaceae)

Evidence-based Complementary and Alternative Medicine (2011) 2011
DOI: 10.1093/ecam/neq023
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Abstract

Vidari is an Ayurvedic herbal drug used as aphrodisiac, galactagogue and is also used in the preparation of Chyavanaprash. Tubers of Ipomoea mauritiana Jacq. (Convolvulaceae), Pueraria tuberosa (Roxb. ex Willd.) DC (Fabaceae), Adenia hondala (Gaertn.) de Wilde (Passifloraceae) and pith of Cycas circinalis L. (Cycadaceae) are all traded in the name of Vidari, creating issues of botanical authenticity of the Ayurvedic raw drug. DNA-based markers have been developed to distinguish I. mauritiana from the other Vidari candidates. A putative 600-bp polymorphic sequence, specific to I. mauritiana was identified using randomly amplified polymorphic DNA (RAPD) technique. Furthermore, sequence characterized amplified region (SCAR) primers (IM1F and IM1R) were designed from the unique RAPD amplicon. The SCAR primers produced a specific 323-bp amplicon in authentic I. mauritiana and not in the allied species. Copyright © 2011 Kambiranda Devaiah et al.

Figures

  • Table 1: Details of the plant samples used in the study.
  • Table 2: Details of the I. mauritiana specific SCAR marker designed from the 600-bp polymorphic sequence.
  • Figure 1: RAPD pattern of Vidari candidates. Lane: 1—Standard DNA marker (100-bp DNA ladder); 2–4—A. hondala; 5–7—C. circinalis; 8–10—P. tuberose; 11–13—I. mauritiana; 14—Standard DNA marker (λ DNA EcoR I/HindIII double digest).
  • Figure 2: Sequence of 600-bp polymorphic region of I. mauritiana, showing binding sites of RAPD (OPA 18) marker and SCAR Marker (IM1F and IM1R).
  • Figure 3: Validation of I. mauritiana specific SCAR marker. Lane: M—Standard DNA marker (100-bp DNA ladder); 1–3— I. mauritiana; 4–6—A. hondala; 7–9—C. circinalis; 10–12—P. tuberose.
  • Figure 4: Diagrammatic representation of RAPD-SCAR marker development to authenticate I. mauritiana Jacq.

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CITATION STYLE

APA

Venkatasubramanian, P., Devaiah, K., & Balasubramani, S. P. (2011). Development of randomly amplified polymorphic DNA based SCAR marker for identification of Ipomoea mauritiana Jacq (Convolvulaceae). Evidence-Based Complementary and Alternative Medicine, 2011. https://doi.org/10.1093/ecam/neq023

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