Development and evaluation of a highly reliable assay for SUMO-specific protease inhibitors

3Citations
Citations of this article
10Readers
Mendeley users who have this article in their library.

Abstract

SUMOylation, as a post-translational modification of proteins, plays essential regulatory roles in a variety of pathological conditions. In the dynamic process of SUMOylation and deSUMOylation, SENPs (SUMO-specific proteases), in charge of deconjugation of SUMO (small ubiquitin-related modifier) from substrate proteins, have recently been found to be potential therapeutic targets for cancer treatment. A reliable and practical assay is much needed to accelerate the discovery of SENPs inhibitors. We established a quantitative assay based on readily available SDS-PAGE-Coomassie system using RanGAP-SUMO as the substrate, thus avoiding the use of expensive fluorescent dyes or the error-prone fluorescent reporter. Its reproducibility and reliability were also evaluated in this report.

Author supplied keywords

Cite

CITATION STYLE

APA

Xie, W., Wang, Z., Zhang, J., Wang, L., Zhao, Y., & Zhou, H. (2016). Development and evaluation of a highly reliable assay for SUMO-specific protease inhibitors. Bioorganic and Medicinal Chemistry Letters, 26(9), 2124–2128. https://doi.org/10.1016/j.bmcl.2016.03.080

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free