Deteksi Gen HptII dan Keragaan Agronomis pada Populasi BC1F1 Tanaman Padi Transgenik

Abstract

Rice varieties tolerant to drought stress are needed to stabilize rice production under drought stress condition. We developed transgenic rice cv. Nipponbare carrying hptII gene that might also contain OsDREB1A gene. OsDREB1A gene responsible to drought tolerance trait need to be transferred into cultivated rice in order to obtain new local rice variety tolerant to drought stress. The aims of this research were to detect the presence of hptII gene in the F1 and BC1F1 transgenic rice and to observe the agronomic performace of those populations and their plant physiology. F1 population was developed by crossing transgenic Nipponbare, as donor parent, with Batutegi, Code, Ciherang, and Konawe genotypes, as recipient parents. BC1F1 population was developed by backcrossing F1 transgenic line with recipient parents, respectively. The presence of hptII gene was analyzed by PCR using a pair of primers for hptII. The observation of agronomic performance was carried out in the green house, meanwhile the observation of stomata was done using microscope. The result of PCR analysis showed that BC1F1 Batutegi trans, BC1F1 Code trans, BC1F1 Konawe trans1, BC1F1 Konawe trans3, dan BC1F1 Konawe trans4 were detected carrying the hptII gene. Agronomic data showed that BC1F1 transgenic rice lines yielded panicles, filled grains, and total grains higher than those of recipent parents. Comparing to the recipient parents, BC1F1 Konawe trans1 and BC1F1 Konawe trans3 had less stomata on the lower side of the leaf, but had more stomata on the upper side of the leaf.