Synthesis and characterization of spion functionalized third generation dendrimers conjugated by gold nanoparticles and folic acid for targeted breast cancer laser hyperthermia: An invitro-assay

Abstract

In this study, 9 nm superparamagnetic iron oxide nanoparticles (SPION) were functionalized by polyamidoamine (PAMAM) dendrimer. Magnetodendrimer samples were conjugated by gold nanoparticles (Au-NPs) using two reducing agents of sodium borohydride and hydrazine sulfate. Presynthesized 10-nm Au-NP were used to evaluate the efficiency of conjugation method. Laser Induced Fluorescence Spectroscopy (LIF) of these materials showed that the nanocomposite and magnetodendrimer have the fluorescence properties covering the whole range of visible spectrum. For targeting and the biocompatibility, the synthesized materials were conjugated by folic acid molecules. Laser-induced hyperthermia using Au-NPs was performed. The samples were characterized using X-ray diffractometry (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, UVVisible spectroscopy, and fluorescent spectroscopy. Two cell lines of breast cancer, i.e. MCF 7 and MDA MB 231, were chosen for cytotoxicity evaluation of the synthesized nanostructures. The Results indicated a high biocompatibility of nanoparticles (up to 50 -gr/mL.