Comparative analysis of genome editors efficiency on a model of mice zygotes microinjection

Abstract

Genome editing is an indispensable tool for functional genomics. The caveat of the ge-nome-editing pipeline is a prevalence of error-prone non-homologous end joining over homologous recombination, while only the latter is suitable to introduce particularly desired genetic variants. To overcome this problem, a toolbox of genome engineering was appended by a variety of improved instruments. In this work, we compared the efficiency of a number of recently suggested improved systems for genome editing applied to the same genome regions on a murine zygote model via microinjection. As a result, we observed that homologous recombination utilizing an ssDNA tem-plate following sgRNA directed Cas9 cleavage is still the method of choice for the creation of animals with precise genome alterations.