Tyrosine phosphorylation based homo-dimerization of arabidopsis RACK1A proteins regulates oxidative stress signaling pathways in yeast

15Citations
Citations of this article
19Readers
Mendeley users who have this article in their library.

Abstract

Scaffold proteins are known as important cellular regulators that can interact with multiple proteins to modulate diverse signal transduction pathways. RACK1 (Receptor for Activated C Kinase 1) is a WD-40 type scaffold protein, conserved in eukaryotes, from Chlamydymonas to plants and humans, plays regulatory roles in diverse signal transduction and stress response pathways. RACK1 in humans has been implicated in myriads of neuropathological diseases including Alzheimer and alcohol addictions. Model plant Arabidopsis thaliana genome maintains three different RACK1 genes termed RACK1A, RACK1B, and RACK1C with a very high (85–93%) sequence identity among them. Loss of function mutation in Arabidopsis indicates that RACK1 proteins regulate diverse environmental stress signaling pathways including drought and salt stress resistance pathway. Recently deduced crystal structure of Arabidopsis RACK1A- very first among all of the RACK1 proteins, indicates that it can potentially be regulated by post-translational modifications, like tyrosine phosphorylations and sumoylation at key residues. Here we show evidence that RACK1A proteins, depending on diverse environmental stresses, are tyrosine phosphorylated. Utilizing site-directed mutagenesis of key tyrosine residues, it is found that tyrosine phosphorylation can potentially dictate the homo-dimerization of RACK1A proteins. The homo-dimerized RACK1A proteins play a role in providing UV-B induced oxidative stress resistance. It is proposed that RACK1A proteins ability to function as scaffold protein may potentially be regulated by the homo-dimerized RACK1A proteins to mediate diverse stress signaling pathways.

References Powered by Scopus

Salt and drought stress signal transduction in plants

5037Citations
N/AReaders
Get full text

Direct analysis of protein complexes using mass spectrometry

2108Citations
N/AReaders
Get full text

When defense pathways collide. The response of arabidopsis to a combination of drought and heat stress 1[w]

1390Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Salicylic acid mediated growth, physiological and proteomic responses in two wheat varieties under drought stress

103Citations
N/AReaders
Get full text

miR393s regulate salt stress response pathway in Arabidopsis thaliana through scaffold protein RACK1A mediated ABA signaling pathways

31Citations
N/AReaders
Get full text

Asc1p/RACK1 connects ribosomes to eukaryotic phosphosignaling

29Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Sabila, M., Kundu, N., Smalls, D., & Ullah, H. (2016). Tyrosine phosphorylation based homo-dimerization of arabidopsis RACK1A proteins regulates oxidative stress signaling pathways in yeast. Frontiers in Plant Science, 7(FEB2016). https://doi.org/10.3389/fpls.2016.00176

Readers over time

‘16‘17‘18‘19‘20‘21‘22‘2502468

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 6

43%

Researcher 6

43%

Professor / Associate Prof. 2

14%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 7

50%

Biochemistry, Genetics and Molecular Bi... 7

50%

Save time finding and organizing research with Mendeley

Sign up for free
0