Tissue and whole-body oxygen uptake in fed and fasted steers

Abstract

The effect of feeding v . fasting, on tissue blood flow, oxygen uptake and proportional contributions of the portal drained viscera (PDV), liver (Expts 1 and 2) and hindquarters (HQ; Expt 2) to whole-body O 2 uptake were studied in beef steers. The combined techniques of indirect calorimetry and net tissue flux, the latter being the product of arterio-venous concentration difference and blood flow, were used in the experiments. In response to fasting, whole-body O 2 consumption decreased as did O 2 uptake by all measured tissues except the liver (trend only in Expt 1). Blood flow to all measured tissues decreased during fasting and fractional uptake of O 2 decreased in PDV and increased in liver and HQ (Expt 2). Proportional contribution of specific tissues to whole-body O 2 uptake changed when animals were switched from the fed to the fasted state. The percentage consumed by PDV decreased from 25.4 to 19.9, by liver increased from 20.5 to 26.4 and by HQ was unchanged (9.6 and 10.5) in Expt 2. These significant responses in Expt 2 were observed as trends in Expt 1. The changes in proportional contribution of tissues to whole-animal O 2 uptake reflect the changing metabolic role of specific tissues to lack of food supply. These findings emphasize the central role of the liver in metabolism and indicate that fasting (catabolic) measurements may not reflect the previous fed (anabolic) physiological state.