Nanohybrid carbon film for electrochemical detection of SNPs without hybridization or labeling

Abstract

(Figure Presented) Base discrimination: A nanocarbon film electrode exhibits sufficiently high activity to obtain sharp, quantitative peaks for all the bases in an oligonucleotide. This property allows the label-free electrochemical analysis of single-nucleotide polymorphisms (SNPs) through the discrimination of single-base differences solely by using electrochemical oxidation (see picture; ssDNA=single-strand DNA). © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.