Multicolor quantum dot-based fluorescence excitation for cellular micro-array screening

Abstract

We have developed a colloidal quantum dot (QD)-based multicolor excitation light source array designed for high throughput lab-on-a-chip cell screening and imaging. We have demonstrated preliminary measurements of fluorescence excitation that is suitable for the arrayed fluorescence immunoassay. Ex vivo transmission microscopy and fluorescence imaging of cultured cancer cells (MDA-MB 231 and SKBr3) were performed to demonstrate the efficacy of the QD-based light source. Our low-cost light source array is highly compatible with commercially available fluorescence microscopes and fluorescent markers commonly used for biomedical imaging and screening.