The rotifer corona by SEM

Abstract

The scanning electron microscope (SEM) is a powerful tool to observe any surface at the ultrastructural level. During the last 15 years, I developed techniques to process rotifer specimens for SEM observation, in order to obtain images of preserved specimens that simulate their natural appearance. A characteristic feature in Rotifera is the rotatory apparatus (corona) and SEM is appropriate for studying its organization. The organization of the corona is better understood if the rotatory apparatus can be examined after the cilia have been removed. A method to prepare the rotifers for observation by SEM is presented.